QIA73
MMP-3 ELISA Kit
Stromelysin-1 ELISA Kit, SL-1 ELISA Kit, Matrix Metalloproteinase 3 ELISA Kit
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
loading
A sandwich ELISA based kit that can be used to assay of MMP-3 in cells in suspension and in adherent cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Protect from Moisture | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | 0.1 ng/ml | ||
| Assay range | 0 - 120 ng/ml | ||
| Assay time | 3 h | ||
| Sample type | Serum or culture media | ||
| Kit contains | 96-Well Coated Plate with strip wells, Sample Diluent, Human MMP-3 Standard, Detector Antibody, HRP Conjugate, Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Declaration | Not available for sale in Japan. | ||
| Store and ship information | |||
|---|---|---|---|
|
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
|||
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Data | |||
|---|---|---|---|
 Levels of MMP-3 (using Cat. No. QIA73) and MMP-9 (using Cat. No. QIA56) were measured following treatment with phorbol 12-myristate 13-acetate (PMA) as outlined in the respective Detailed Protocol.  Levels of MMP-3 were measured in MG-63 cells following treatment with PMA for 96 h as outlined in the Detailed Protocol.  The lower limit of detection was measured by assaying three replicates of zero four times using two different lots of plates and two different lots of detector antibody. The grand mean signal and pooled standard deviation of zero was calculated. The grand mean of each standard (run in replicates of three in the four assays) was used for the standard curve, and the response, mean signal of zero plus two standard deviations, read in dose from the standard curve is the LLD; that is, the smallest dose that is not zero with 95% confidence.  Levels of MMP-3 in human serum (top) and cell culture supernatants of CCF-STTG1 astrocytoma cells (bottom) were measured following immunaffinity extraction with three MMP-3 antibodies (that are not used in this ELISA kit) and TIMP antibodies. PBS was used as the no-antibody control.  The levels of human MMP-3/TIMP complex (8 ng/ml; purified protein) and MMP-3 (8 ng/ml; purified protein) were measured as outlined in the Detailed Protocol. |
