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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Teléfono: +49 6151 72-0
Fax: +49 6151 72 2000
15 febrero 2012
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The LIC Duet™ Trx•Tag™ Ek Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), ATG translation initation codon, and an N-terminal Trx•Tag coding sequence. The Trx•Tag is a 109 aa sequence that encodes thioredoxin. Fusion to this sequence has been demonstrated to enhance solubility of many proteins that are typically insoluble in E. coli (1-2).
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