CBA003
InnoZyme™ Gelatinase (MMP-2/MMP-9) Activity Assay kit, Fluorogenic
MMP-2/MMP-9 Activity Assay Kit, Fluorogenic
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
loading
A sensitive fluorogenic assay (Exc. max: ~325 nm; Emi. max.: ~393 nm) for the measurement of gelatinases (MMP-2 and MMP-9). Also useful for the screening of gelatinase inhibitors.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorogenic | ||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | 20 ng/ml | ||
| Assay time | 2 - 6 h | ||
| Sample type | Cell lysate, culture supernatant, tissue extract, plasma, serum, purified enzyme | ||
| Positive control | MMP-9 (Cat. No. PF140) | ||
| Kit contains | Gelatinase Substrate, pro-MMP-2 Positive Control, Inhibitor, APMA, Assay Buffer, 96-Well Plate, Plate Sealer, and a user protocol | ||
| Store and ship information | |||
|---|---|---|---|
|
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
|||
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Data | |||
|---|---|---|---|
 Human Pro-MMP-2 (supplied) was activated as outlined in the Reagent Preparation section above and assayed for activity as outlined in the Detailed Protocol, incubating for a total of 3 h.  Activated MMP-9 (Cat. No. PF140) was assayed without APMA at 37°C for 3 h according to the Detailed Protocol outlined above.  Serum from 5 different donors was diluted 1:5 in Activation Buffer and HT1080 culture supernatant was diluted 1:3 with Activation Buffer. All samples were incubated at 37°C for 3 h according to the Detailed Protocol outlined above. |
