70796
His•Tag® Monoclonal Antibody
Sensitive, specific detection of His•Tag fusion proteins
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1-0.2 µg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-µg package size provides enough purified antibody for 1000 ml of working solution.
* Not recommended for HRP-based detection of Trail Mix Western Markers.
| Related products | |
|---|---|
| 70972 | His•Tag® AP Western Reagents |
| 70973 | His•Tag® AP LumiBlot Reagents |
| 70974 | His•Tag® HRP LumiBlot Reagents |
| Related products for His•Tag® Monoclonal Antibody | |
| 70972 | His•Tag® AP Western Reagents |
| 70973 | His•Tag® AP LumiBlot Reagents |
| 70974 | His•Tag® HRP LumiBlot Reagents |
| Store and ship information | |||
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| Storage | +2°C to +8°C | ||
| Ship |
Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling |
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