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15 febrero 2012
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The HDAC Activity Assay Kit is designed to measure HDAC activity in cell or nuclear extracts, immunoprecipitates or purified enzymes.
| Información sobre producto | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Avoid freeze/thaw | Si | ||
| Sample type | cell extracts, immunoprecipitates, or purified enzymes | ||
| Almacenar y enviar información | |||
|---|---|---|---|
| Categoría de alamacenamiento | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Información de seguridad | |||
|---|---|---|---|
| Frase S |
S: 26-36-45 |
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| Frase R |
R: 36/38 |
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| Datos | |||
|---|---|---|---|
 <b><sup>1</sup></b>Refers to dilution of Trichostatin A in HDAC Assay Buffer, which will be 5X the final concentration. Examples: 1) As a measure of non-HDAC background, 5 µM would produce final 1 µM concentration and essentially complete HDAC inhibition; 2) As a model inhibitor "hit", 25 nM would produce final 5 nM and ~50% inhibition. <b><sup>2</sup></b> Refers to dilution of potential inhibitor in Assay Buffer, which will be 5x its final concentration.  <b><sup>1</sup></b>The appropriate dilution of the Deacetylated Standard may be determined from the standard curve and should be the concentration producing a fluorescent signal equal to that produced by control (no inhibitor) samples in the HDAC assay. The dilution in HDAC Assay Buffer is prepared at 1.25X this concentration to compensate for the 4/5 dilution due to addition of 10 µl of Assay Buffer or inhibitor. <b><sup>2</sup></b> Refers to dilution of Trichostatin A in HDAC Assay Buffer, which will be 5X its final concentration in the 50 µl volume, prior to addition of HDAC Developer WS. Example: As a model inhibitor that does not interfere with the HDAC Developer, 25 nM Trichostatin A would produce a final 5 nM concentration. <b><sup>3</sup></b>Refers to dilution of potential inhibitor in HDAC Assay Buffer, which will be 5X its final concentration in the 50 µl volume, prior to addition of HDAC Developer WS.   50 µl of each diluted Deacetylated Standard was mixed with 50 µl HDAC Developer and incubated for 10 min at 25°C. Fluorescence was then measured using the clear 1/2 Volume Plate with a fluorimeter (PerSeptive Biosystems, Ex. 360 nm, Em. 460 nm, gain=85) |
