For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0. | ||
| Molar mass | 51632 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥100 units/µg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0. | ||
| EC number | 3.4.21.78 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
||
