69001
First Strand cDNA Synthesis Kit
Reliable preparation of templates for RT-PCR
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
14 February 2012
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The First Strand cDNA Synthesis Kit is designed for the preparation of high quality first strand cDNA from cellular RNA templates. The kit contains MMLV Reverse Transcriptase for superior yields of full-length cDNA. Both oligo(dT) and random hexamer primers are included for a choice of general priming strategies and as alternatives to user-supplied specific primers. A small volume (1-2 µl) of the first strand cDNA reaction can be used in PCR amplification with KOD Hot Start or KOD XL DNA Polymerase. Use this kit in conjunction with the Straight A’s™ mRNA Isolation System and appropriate PCR reagents to amplify rare coding regions.
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| Avoid freeze/thaw | Yes | ||||||||||||||||||||||||||||||||
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| Storage | Multiple storage requirements | ||
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Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
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| S Phrase |
S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 22-36/38 Harmful if swallowed. Irritating to eyes and skin. |
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| Data | |||
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 Figure 1. The positive control RNA was subjected to first strand cDNA synthesis with various primer combinations followed by addition of the 5'-sense Control Primer (and antisense Control Primer, where appropriate) and amplification. First strand primers are indicated. AS: antisense Control Primer, RH: random hexamers, dT: oligo (dT). |
