Unit definition: One unit of Restriction Grade Factor Xa cleaves 50 μg Xa Cleavage Control Protein to > 95% completion in 16 hours at 21°C in a buffer containing 50 mM Tris-HCl, 100 mM NaCl, and 5 mM CaCl2, pH 8.0.
Restriction Grade Factor Xa is a highly purified preparation isolated from bovine plasma and activated with Russell's viper venom. This preparation is purified to near homogeneity and shows no secondary cleavage from contaminating proteases. The preparation is also functionally tested for activity with fusion proteins. Like enterokinase, Factor Xa cleaves at the C-terminal side of the recognition sequence (IleGluGlyArg↓) and can therefore be used for removing all vector-encoded sequences from appropriately designed constructs.
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Produktinformationen
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Avoid freeze/thaw
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Ja
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Unit definition
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One unit of enzyme cleaves 50 µg Xa Cleavage Control Protein to > 95% completion in 16 hours at 21°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl2.
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Components
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| • | 400 U | Restriction Grade Factor Xa
| | • | 2 ml | Factor Xa Dilution/Storage Buffer
| | • | 1 ml | 10X Factor Xa Cleavage Buffer
| | • | 10 µg | Cleavage Control Protein |
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Lager- und Versandinformationen
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Lagerkategorie
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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