69182
DNase I, RNase free
RNase-free DNase I
For applications in which maintenance of RNA integrity is critical
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation. For applications requiring the use of DNase I for random double-stranded cleavage of DNA, try DNase I, ds Qualified.
| Related products | |
|---|---|
| 69164 | DNase I, ds Qualified |
| Related products for DNase I, RNase free | |
| 69164 | DNase I, ds Qualified |
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl2, 10 mM DTT, 1 µg plasmid DNA, and enzyme. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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