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Merck KGaA
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15 February 2012
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DNase I, ds Qualified is a special preparation
of bovine pancreatic DNase I that can be
used for double-stranded cleavage of DNA
molecules in preparation for cloning or other
applications. In the presence of Mn2+ and
absence of Mg2+, the enzyme makes double-strand
breaks at random sites in DNA. The
average size of the resulting fragments can
be controlled by the enzyme concentration
and incubation time. This method is useful for
the creation of shotgun libraries and is used
in the NovaTope® System to prepare random
subclones of target genes for functional
domain analysis. The enzyme also is included
in the DNase Shotgun® Cleavage Kit, which
contains reaction buffer and MnCl2 solution.
For applications requiring general cleavage
or degradation of DNA, please see RNase-free
DNase I and Benzonase® Nuclease.
| Related products | |
|---|---|
| 69182 | DNase I, RNase free |
| Related products for DNase I, ds Qualified | |
| 69182 | DNase I, RNase free |
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit will degrade 1 µg DNA in 10 minutes at 37°C. The 50-µl reaction mixture contains 80 mM HEPES, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 10 mM DTT, 1 µg plasmid DNA, and enzyme. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
||
