For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
loading
Cre Recombinase catalyzes the site-specific
recombination of DNA between 34-bp
loxP sites derived from bacteriophage P1
(Sternberg 1986). The enzyme is a Type I
topoisomerase that requires no cofactors or
energy to effect recombination. The type of
recombination event depends on the relative
orientation of the two loxP sites. Tandemly
repeated sites cause circularization of the
DNA between them, making possible the
preparation of plasmid subclones from larger
DNA (e.g., Novagen lambda cloning vectors).
If the loxP sites are in opposite orientations,
the DNA between them is inverted by Cre
Recombinase. Two DNA molecules having
single loxP sites are joined by the enzyme,
making this system useful for targeting
sequences into large genomes (Fukeshige
1992, Baubonis 1993). Because the enzyme
uses no energy, Cre Recombinase reactions
result in an equilibrium that does not favor
the accumulation of one product over another.
Novagen Cre Recombinase is highly purified
from a recombinant source, and is free of
contaminating nucleases. It is qualified for in
vitro applications, including circularization
of plasmids out of loxP-containing lambda
vectors. A loxP Control DNA and 10X reaction
buffer are included.
| Product information | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Avoid freeze/thaw | Yes | |||||||||||
| Unit definition | One unit is defined as the amount of enzyme required to achieve maximal specific site recombination of 0.5 µg test DNA in one hour at 37°C in a 30-µl reaction volume. | |||||||||||
| Components |
|
|||||||||||
| Contaminants | Free of contaminating nucleases. | |||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
