70573
Clonables™ 2X Ligation Premix
One solution for optimal ligation in 15 minutes
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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The Clonables™ 2X Ligation Premix is a single solution containing optimized concentrations of the highest quality T4 DNA ligase, buffer, stabilizer, and cofactors needed for efficient ligation of any type of compatible DNA ends. The premix is tested for ligation of compatible 2- to 4-base cohesive DNA ends as well as blunt ends and single-base overhangs found on some PCR products. Under most conditions
excellent ligation is achieved in only 15 minutes; with T/A or U/A overhangs more colonies can be obtained by incubating up to two hours. Although maximal efficiencies are obtained using NovaBlue Singles Competent Cells, this reagent is compatible with transformation of any type of chemically competent cells provided by the user. The high performance, minimal pipetting requirement, and single-addition format make the premix suitable for high-throughput applications.
One vial of the 2X Ligation Premix (Cat. No. 70573-3) contains enough reagent for 11 ligation reactions (10-µl scale).
One vial of the 2X Ligation Premix (Cat. No. 70573-3) contains enough reagent for 11 ligation reactions (10-µl scale).
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Irritant |
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