BLT5615rna is a derivative of the BLT5615 host strain in which the RNase I gene (rna) has been disrupted by insertion of a kanamycin resistance cassette. RNA is almost completely stable in phage lysates of BLT5615rna, making this an ideal host for T7 phage display studies of both magnesium-dependent and magnesium-independent RNA-protein interactions (1).
BLT5615rna carries an ampicillin-resistant plasmid that supplies large amounts of capsid protein 10A. Growth of T7Select10-3, T7Select1-1 and 1-2 clones on BLT5615rna requires the addition of IPTG to induce production of the 10A protein. BLT5615rna is a less inhibitory host than BLT5403, where 10A expression is controlled by a lacUV5 promoter.
| Related products | |
|---|---|
| 69142 | BLT5403 Glycerol Stock |
| 69905 | BLT5615 Glycerol Stock |
| Related products for BLT5615rna Glycerol Stock | |
| 69142 | BLT5403 Glycerol Stock |
| 69905 | BLT5615 Glycerol Stock |
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling |
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