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15 February 2012
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When used with Novagen's Streptavidin Alkaline Phosphatase Conjugate, Biotinylated T7•Tag Antibody enables a 5-fold increase in detection sensitivity of target proteins on Western blots compared to an anti-IgG conjugate strategy. This form of the antibody also enables specific detection in the presence of other mouse IgGs.
The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).
This antibody is also available as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
| Specificity | T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal |
| Species/Isotype | Mouse monoclonal IgG2b |
| Cross-reactivity | Negligible with bacterial, insect or mammalian cell lysates |
| Sensitivity | 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (developed with chemiluminescent substrates) |
| Form | Stabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein |
| Working dilution | 1:5,000–1:10,000 |
| Related products | |
|---|---|
| 69048 | T7•Tag® Antibody HRP Conjugate |
| 69522 | T7•Tag® Monoclonal Antibody |
| 69999 | T7•Tag® Antibody AP Conjugate |
| Related products for Biotinylated T7•Tag® Monoclonal Antibody | |
| 69048 | T7•Tag® Antibody HRP Conjugate |
| 69522 | T7•Tag® Monoclonal Antibody |
| 69999 | T7•Tag® Antibody AP Conjugate |
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| Avoid freeze/thaw | Yes | ||||||||
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| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |
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