This kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test.
Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator.
| Product information | |||
|---|---|---|---|
| Format | Fluorescence microscopy | ||
| Form | 100 Tests | ||
| Detection method | Fluorescence | ||
| Species reactivity | a broad range of species | ||
| Protect from Moisture | Yes | ||
| Assay time | 0.5 h | ||
| Sample type | Cell suspensions or adherent cells | ||
| Kit contains | Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36/37/39-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 36/37/38-46 May cause heritable genetic damage. |
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