A highly sensitive, reliable cell proliferation assay utilizing Calcein-AM, a fluorescent probe for staining viable cells (Ex.: ~485 nm; Em.: ~520 nm). For optimal results serum-free media or Dulbecco’s PBS is recommended. Endogenous esterases in serum can lead to increased blank values which can be subtracted out. Extremely useful for cytotoxicity assays.
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Product information
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Format
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96-well plate
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Form
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500 Tests
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Detection method
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Fluorometric
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Protect from Light
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Yes
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Sensitivity
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100 Cells
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Assay range
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250-25,000 cells
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Assay time
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1 h
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Sample type
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Viable cells
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Kit contains
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Calcein-AM, Dulbecco-PBS.
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Store and ship information
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Storage
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-20°C
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Ship
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Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS
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