Recognizes the ~60 kDa Smad1/5 protein phosphorylated at Ser463/465 in HeLa cells treated with BMP-4.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 463/465 phosphorylation sites of human Smad5, coupled to KLH. | ||
| Clone | 41D10 | ||
| Form | Liquid | ||
| Formulation | In 50% glycerol, 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, pH 7.5. | ||
| Species reactivity | human, mouse | ||
| Preservative | ≤0.1 sodium azide | ||
| Applications |
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| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
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 Detection of human Smad1/5, phosphorylated at pSer<sup>463/465</sup>, by immunoblotting. Samples: whole cell lysates from HeLa or NIH/3T3 cells, left untreated or treated with BMP-4, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer<sup>463/465</sup>) Rabbit mAb (41D10) (Cat. No. PS1024) (1:1000). Detection: chemiluminescence.  Detection of human Smad1/5 phosphorylated at pSer<sup>463/465</sup>, by flow cytomtry. Samples: HeLa cells left untreated (blue) or treated with BMP-4 (green). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer<sup>463/465</sup>) Rabbit mAb (41D10) (Cat. No. PS1024) (1:200) (blue and green) and a nonspecific, negative control antibody (red). Detection: fluorescence. |
