Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH. | ||
| Clone | C25A9 | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human Smad3, phosphorylated at Ser<sup>423/425</sup>, by immunoblotting. Samples: whole cell lysate from HT-1080 cells, left untreated or treated with TGF-β1, TGFR inhibitor (SB-431542), or BMP-2, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad3 (pSer<sup>423/425</sup>) Rabbit mAb (C25A9) (Cat. No. PS1023) (1:1000) (top panel) and Anti-Smad3 (total) (bottom panel). Detection: chemiluminescence. |
