For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
14 February 2012
loading
Recognizes the ~220 kDa BRCA1 protein phosphorylated at Ser1524.
| Alternatives | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| Alternative products for PhosphoDetect™ Anti-BRCA1 (pSer¹524) Rabbit pAb | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| Accessories forPhosphoDetect™ Anti-BRCA1 (pSer¹524) Rabbit pAb |
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹524 phosphorylation site of human BRCA1 | ||
| Form | Liquid | ||
| Formulation | In Tris-citrate/phosphate buffer, pH 7.0-8.0. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
|
||
| Positive control | Irradiated HeLa cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
| Data | |||
|---|---|---|---|
 Detection of human BRCA1, phosphorylated on Ser<sup>1524</sup>, by immunoblotting. Samples: Nuclear extract of HeLa cells mock irradiated (lane 1) or irradiated with 10 Gy of ionizing radiation (lane 2). Primary antibody: PhosphoDetect™ Anti-BRCA1 (pSer<sup>1524</sup>) Rabbit pAb (Cat. No. PC732) (1:5000). Detection: chemiluminescence. |
