Recognizes the ~220 kDa BRCA1 protein phosphorylated at Ser1524.
| Accessories for PhosphoDetect™ Anti-BRCA1 (pSer¹5²4) Rabbit pAb |
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Product information
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Host
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Rabbit
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Isotype
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IgG
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Immunogen
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a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹5²4 phosphorylation site of human BRCA1
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Form
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Liquid
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Formulation
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In Tris-citrate/phosphate buffer, pH 7.0-8.0.
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Species reactivity
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human
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Preservative
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≤0.1% sodium azide
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Applications
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Immunoblotting (Western Blotting)
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Positive control
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Nuclear extracts from irradiated HeLa cells
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Store and ship information
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Storage
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+2°C to +8°C
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Ship
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Blue or Dry Ice
Standard Handling
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Data
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Detection of human BRCA1, phosphorylated on Ser<sup>1524</sup>, by immunoblotting. Samples: Nuclear extract of HeLa cells mock irradiated (lane 1) or irradiated with 10 Gy of ionizing radiation (lane 2). Primary antibody: PhosphoDetect™ Anti-BRCA1 (pSer<sup>1524</sup>) Rabbit pAb (Cat. No. PC732) (1:5000). Detection: chemiluminescence.
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