Recognizes the ~21 kDa p21WAF1 protein in skin or colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
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Product information
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Host
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Mouse
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Isotype
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IgG1
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Immunogen
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full-length, recombinant human p21WAF1
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Epitope
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within amino acids 58-77 of human p21WAF1
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Clone
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DF10
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Form
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Liquid
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Formulation
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In 0.05 M sodium phosphate buffer, 0.2% gelatin.
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Species reactivity
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human, not mouse, not rat
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Preservative
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≤0.1% sodium azide
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Applications
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Immunoblotting (Western Blotting)
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Immunoprecipitation
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Do not freeze
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Yes
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Positive control
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Any cell line expressing wild-type p53 (e.g. Hs27 or U2Os cells treated with DNA-damaging agents)
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Store and ship information
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Storage
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+2°C to +8°C
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Ship
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Blue or Dry Ice
Standard Handling
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