Recognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | full-length, recombinant, human p21WAF1 | ||
| Epitope | within amino acids 58-77 of human p21WAF1 | ||
| Clone | EA10 | ||
| Form | Liquid | ||
| Formulation | In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. | ||
| Species reactivity | human, not mouse, not rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Do not freeze | Yes | ||
| Positive control | Any cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue | ||
| Negative control | Unstimulated cells, unstimulated skin tissue, or SK-OV-3 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of p21 WAF1 by immunoblotting: Sample: Whole cell lysate (56 µg) from MCF-7 cells. Primary antibody: Anti-p21<sup>WAF1</sup> (Ab-1) Mouse mAb (EA10) (Cat. No. OP64) (3 µg/ml). Secondary antibody: Anti-Mouse IgG (Goat) Peroxidase Conjugate. Detection: chemiluminescence. |
