Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹7 phosphorylation site of human Chk1 | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | ||
| Species reactivity | human, monkey, mouse, rat | ||
| Preservative | None | ||
| Applications |
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| Positive control | Lysates from COS cells treated with UV | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human and monkey Chk1, phosphorylated at Ser<sup>317</sup>, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer<sup>317</sup>) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence. |
