Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
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Product information
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Host
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Rabbit
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Isotype
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IgG
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Immunogen
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a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹7 phosphorylation site of human Chk1
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Form
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Liquid
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Formulation
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In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
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Species reactivity
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human, monkey, mouse, rat
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Preservative
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None
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Applications
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Immunocytochemistry
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Immunoblotting (Western Blotting)
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Paraffin Sections
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Positive control
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Lysates from COS cells treated with UV
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Store and ship information
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Storage
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-20°C
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Ship
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Blue or Dry Ice
Standard Handling
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Data
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 Detection of human and monkey Chk1, phosphorylated at Ser<sup>317</sup>, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer<sup>317</sup>) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
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