Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form in 293T and A431 cell nuclear extracts.
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Product information
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Host
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Rabbit
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Isotype
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IgG
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Immunogen
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a synthetic peptide corresponding to amino acids at the C-terminus of human H2AX
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Form
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Liquid
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Formulation
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In Tris/citrate-phosphate buffer, pH 7-8.
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Species reactivity
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human, mouse
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Preservative
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≤0.1% sodium azide
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Applications
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Immunoblotting (Western Blotting)
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Immunoprecipitation
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Positive control
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Nuclear extracts from human 293T or G-361 cells
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Store and ship information
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Storage
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+2°C to +8°C
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Ship
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Blue or Dry Ice
Highly Toxic
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Data
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 Detection of human H2AX by immunoblotting. Sample: Nuclear extract from HEK293T cells, H2AX knockout (-/-), H2AX wild-type (+/+), and G-361 cells. Primary antibody: Anti-H2AX Rabbit pAb (Cat. No. DR1016) (1:1000). Detection: chemiluminescence.
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