A sensitive and robust homogeneous sphingosine kinase assay that does not require radioactive materials or secondary (detector) enzymes or antibodies. The kit is ideally suited for automated screening (HTS) and can be read on any fluorimeter.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 100 Tests | ||
| Assay time | 2 h | ||
| Sample type | Purified enzyme | ||
| Kit contains | Assay Buffer, Coordination Buffer, Sensor Stock, Sensor Dilution Buffer, Trivalent Metal Ion, Post Reaction Buffer, and a user protocol. | ||
| Store and ship information | |||
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | +2°C to +8°C | ||
| Ship |
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 16-36/37-45-7-A09 Keep away from sources of ignition - No Smoking. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). Keep container tightly closed. |
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| R Phrase |
R: 10-23/24/25-39/23/24/25 Flammable. |
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| Data | |||
|---|---|---|---|
   Varying concentrations of enzyme were mixed with 1 µM substrate in cAB containing 100 µM ATP. Relative fluorescence units (RFU) decrease as a function of zyme activity.  Varying amounts of ATP were used in the absence or presence of enzyme. Percent activity was calculated based on the ΔRFU between samples.  Varying amounts of DESPH in 1% DMSO were added to enzyme (30 nM) and substrate (1 µM) in cAB containing 50 µM ATP.  Statistical data were produced using 0% (top) 100% phospho-calibrators (bottom) or 30 nM sphingosine kinase (middle). Z′-factors of 0.9 and 0.6 were obtained using phospho-calibrators or reacted samples, respectively. A Z′-factor of >0.5 indicates a robust assay. |
