A sensitive and robust homogeneous PI 3-K assay that does not require radioactive materials or secondary (detector) enzymes or antibodies. The kit is ideally suited for automated screening (HTS) and can be read on any fluorimeter.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 100 Tests | ||
| Assay time | 2 h | ||
| Sample type | Purified enzyme | ||
| Kit contains | Assay Buffer, Coordination Buffer, Sensor Stock, Sensor Dilution Buffer, Trivalent Metal Ion, Post Reaction Buffer, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 1/2-36/37-45-63-A09 Do not keep the container sealed. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). In case of accident by inhalation: remove casualty to fresh air and keep at rest. |
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| R Phrase |
R: 10-23/24/25-39/23/24/25 Flammable. |
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| Data | |||
|---|---|---|---|
   Varying concentrations of enzyme were mixed with 1 µM substrate in cAB containing 75 µM ATP. Relative fluorescence units (RFU) decrease as a function of enzyme activity.  Varying amounts of ATP were used in the absence or presence of enzyme. Percent activity was calculated based on the ΔRFU between the samples.  Varying amounts of Wortmannin in 0.05% DMSO were added to enzyme (32 nM) and substrate (1 µM) in cAB containing 20 µM ATP.  Statistical data were produced using 0% (top) and 100% phospho-calibrators (bottom) or 27 nM PI 3-Kα (middle). Z′-factors of 0.8 and 0.7 were obtained using phospho-calibrators or reacted samples, respectively. A Z′-factor of >0.5 indicates a robust assay. |
