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Merck KGaA
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14 February 2012
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Detects and quantifies the level of PRAS40 (Proline-Rich AKT Substrate of 40 kDa) protein that is phosphorylated at Thr246 in mouse, human, and rat samples. PRAS40 is a 14-3-3 binding protein that is a direct substrate of Akt. It is believed to influence protein interactions, nuclear transport, and enzyme activities.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Sensitivity | < 0.9 units/ml | ||
| Assay range | 1.6-100 Units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysate, tissue extract | ||
| Positive control | 3T3L1 cells | ||
| Kit contains | PRAS40 (pThr246) Standard, Standard Diluent Buffer, PRAS40 Antibody-Coated 96-Well Plate, Rabbit Anti-PRAS40 (pThr246) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of PRAS40 (pThr<sup>246</sup>). The data presented in Figure 1 show that the sensitivity of the ELISA is ~2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using rabbit anti-PRAS40 (pThr<sup>246</sup>) and an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent substrate and autoradiography detection. |
