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CBA067  PhosphoDetect™ PRAS40 (pThr²46) ELISA Kit

Detects and quantifies the level of PRAS40 (Proline-Rich AKT Substrate of 40 kDa) protein that is phosphorylated at Thr246 in mouse, human, and rat samples. PRAS40 is a 14-3-3 binding protein that is a direct substrate of Akt. It is believed to influence protein interactions, nuclear transport, and enzyme activities.
Product number Size Quantity Price
CBA067-1KIT  1 kit  This Product is currently discontinued or not available.
Product information
Format 96-well plate
Form 96 Tests
Detection method Colorimetric
Species reactivity human, mouse, rat
Sensitivity < 0.9 units/ml
Assay range 1.6-100 Units/ml
Assay time 4 h
Sample type Cell lysate, tissue extract
Positive control 3T3L1 cells
Kit contains PRAS40 (pThr²46) Standard, Standard Diluent Buffer, PRAS40 Antibody-Coated 96-Well Plate, Rabbit Anti-PRAS40 (pThr²46) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.
Store and ship information
Storage +2°C to +8°C
Ship Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Data

The sensitivity of this ELISA was compared to immunoblotting using known quantities of PRAS40 (pThr<sup>246</sup>). The data presented in Figure 1 show that the sensitivity of the ELISA is ~2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using rabbit anti-PRAS40 (pThr<sup>246</sup>) and an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent substrate and autoradiography detection.

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