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14 February 2012
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Detects and quantifies the level of ATF2 (activating transcription factor 2) protein that is phosphorylated at Thr69/71. ATF2 is a member of the cAMP response element-binding protein family of basic region-leucine zipper proteins, which is implicated in growth control, cell cycle progression, differentiation, and transformation. ATF2 is a transcription factor that regulates the expression of genes containing a cAMP-response element. Although this kit is designed for human cell lines, platelets, and lymphocytes, it cross-reacts with mouse and rat cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Unit definition | One unit (U) is defined as the amount of ATF2 (pThr69/71) derived from 2.5 ng of ATF2 that was phosphorylated by active JNK2. | ||
| Sensitivity | <1.0 U/ml | ||
| Assay range | 1.56-100 U/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Kit contains | ATF2 (Thr69/71) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated Microtiter Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of ATF2 (pThr<sup>69/71</sup>). The data presented in Figure 1 show that the sensitivity of the ELISA is ~8x greater than that of immunoblotting. The bands shown in the immunoblotting data were developed using an anti ATF2 (pThr<sup>69/71</sup>) antibody and chemiluminescent detection. |
