Detects the dually phosphorylated forms of JNK1 and JNK2 at Thr183 and Tyr185 in human samples. This activation occurs via a variety of signals including growth factors, inflammatory cytokines, and environmental stress.
| Accessories for PhosphoDetect™ JNK1/2 (pThr¹8³/pTyr¹85) ELISA Kit |
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Product information
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Format
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96-well plate
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Form
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96 Tests
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Detection method
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Colorimetric
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Species reactivity
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human
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Avoid freeze/thaw
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Yes
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Sensitivity
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≤0.8 units/ml
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Assay range
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1.6-100 units/ml
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Assay time
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4 h
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Sample type
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Cell lysates
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Kit contains
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Coated 96-Well Plate, JNK1/2 Phospho-Thr¹8³/Tyr¹85 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.
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Store and ship information
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Storage
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+2°C to +8°C
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Ship
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Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS
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Canadian export regulations
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Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
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Data
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The analytical sensitivity of this assay is less than 0.8 units/ml of human phospho-JNK1/2 (Thr<sup>183</sup>/Tyr<sup>185</sup>). This was determined by adding two standard deviations to the mean Abs obtained when the zero standard was assayed 30 times. The sensitivity of this ELISA as compared to immunoblotting using known quantities of phospho-JNK1/2 (Thr<sup>183</sup>/Tyr<sup>185</sup>), (figure 1), shows that the ELISA is ~2X more sensitive than that of immunoblotting. The bands shown in the Western blot were developed using Anti-SAPK/JNK, Phospho-Specific (Thr<sup>183</sup>, Tyr<sup>185</sup>) Human (Rabbit), Cat. No. 559309, and an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent substrate and autoradiography.
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