Detects Akt1 when phosphorylated on Ser473. Does not detect phosphorylated Akt3. Cross-reactivity with phosphorylated Akt2 has not been thoroughly evaluated. Akt is activated by a variety of stimuli including insulin through phosphorylation on Thr308 and Ser473. Once phosphorylated Akt migrates to the nucleus where it is involved in a variety of cellular processes such as the transduction of signals that prevent apoptosis. Although this kit was developed for human samples, it has been found to cross react with mouse and rat.
| Accessories for PhosphoDetect™ Akt (pSer47³) ELISA Kit |
|
Product information
|
|
Format
|
96-well plate
|
|
Form
|
96 Tests
|
|
Detection method
|
Colorimetric
|
|
Species reactivity
|
human, mouse, rat
|
|
Avoid freeze/thaw
|
Yes
|
|
Unit definition
|
One unit is defined as the amount of Akt (pSer47³) derived from 100 pg of Akt, which was phosphorylated by MAPKAP2 and PDK1.
|
|
Sensitivity
|
≤0.8 units/ml
|
|
Assay range
|
1.6-100 units/ml
|
|
Assay time
|
4 h
|
|
Sample type
|
Cell lysates
|
|
Positive control
|
Akt (pSer47³)
|
|
Kit contains
|
Coated 96-Well Plate, Akt Phospho-Ser47³ Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol.
|
|
Store and ship information
|
|
|
|
Storage
|
+2°C to +8°C
|
|
Ship
|
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS
|
|
Safety information
|
|
S Phrase
|
S: 26-36/37-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
|
|
R Phrase
|
R: 21/22-36/38
|
