The pCDF-2 Ek/LIC Vector is a LIC-compatible derivative of pCDF-1b. The plasmid contains a strong T7lac promoter and an amino terminal His•Tag® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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Product information
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Components
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| • | 1 µg | Ek/LIC Vector
| | • | 25 units | T4 DNA Polymerase, LIC-qualified
| | • | 50 µl | 25 mM EDTA
| | • | 40 µl | 25 mM dATP
| | • | 8 µl | Ek/LIC β-gal Control Insert
| | • | 100 µl | 100 mM DTT
| | • | 50 µl | 10X T4 DNA Polymerase Buffer
| | • | 1.5 ml | Nuclease-free Water
| | • | 5 × 2 ml | SOC Medium
| | • | 10 µl | Test Plasmid
| | • | 0.2 ml | BL21(DE3)pLysS Competent Cells
| | • | 0.2 ml | BL21(DE3) Competent Cells
| | • | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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Licence Brookhaven
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This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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