Directional PCR cloning into the most powerful E. coli
expression vectorsThe pET-43.1 series of vectors are designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the Nus•Tag fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (
TB318). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
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Product information
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Components
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| • | 1 µg | Ek/LIC Vector
| | • | 10 µl | Ek/LIC Control Insert
| | • | 25 U | T4 DNA Polymerase, LIC-qualified
| | • | 50 µl | 10X T4 DNA Polymerase Buffer
| | • | 100 µl | 100 mM DTT
| | • | 50 µl | 25 mM EDTA
| | • | 40 µl | 25 mM dATP
| | • | 1.5 ml | Nuclease-free Water
| | • | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells
| | • | 0.2 ml | BL21(DE3) Competent Cells
| | • | 0.2 ml | BL21(DE3)pLysS Competent Cells
| | • | 5 × 2 ml | SOC Medium
| | • | 10 µl | Test Plasmid |
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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Licence Brookhaven
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This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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