Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F– ompT hsdSB (rB– mB–) gal dcm (DE3) pLacIRARE (CamR)
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Product information
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Components
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| 0.4 ml | 1 ml | Component
| | • | 2 × 0.2 ml | 5 × 0.2 ml | Rosetta(DE3)pLacI Competent Cells
| | • | 2 × 2 ml | 4 × 2 ml | SOC Medium
| | • | 2 ng | 2 ng | Test Plasmid |
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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Licence Brookhaven
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This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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