The pTriEx™-4 Ek/LIC vector is an Ek/LIC version of pTriEx-4. The pTriEx-4 Ek/LIC vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, the pTriEx-4 Ek/LIC vector contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. The pTriEx-4 Ek/LIC vector contains N-terminal His•Tag^® and S•Tag™ coding sequences followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag coding sequence. This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.