The pLacI plasmid is a chloramphenicol resistant plasmid that encodes the
lac repressor protein. The p15a origin of replication is compatible with colE1 based vectors, including Novagen's pETBlue™ and pTriEx™ plasmids. These expression vectors contain
lac operators to regulate T7 promoter-driven expression, but do not supply the
lac repressor. Thus co-transformation with pLacI supplies a source of
lac repressor protein to maintain repression of these vectors in λDE3 lysogenic bacterial expression host strains. Expression of
lacI is driven by a constitutive
E. coli promoter. Unique restriction sites are indicated on the vector map (
TB269).
