The Clonables™ Kit enables convenient,
dependable, high-efficiency ligation and
transformation of any compatible DNA
ends. The kit features a unique, universal
Ligation Premix, containing ligase, buffer, and
cofactors, which supports ligation of any type
of DNA cohesive or blunt ends in a 15-minute
reaction. Ligated DNA is transformed into
NovaBlue Singles™ Competent Cells, which
use a streamlined protocol that takes less than
8 minutes for ampicillin-resistant plasmids
and 38 minutes for other antibiotic-resistant
plasmids. This kit can be used with a variety
of cloning vectors and is compatible with any
type of DNA end, without altering the ends or
desired cloning junctions.
The Clonables Kit contains sufficient
reagents to perform 11 ligation and
transformation reactions, and includes a
control vector and insert mix to verify
performance.
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Product information
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Avoid freeze/thaw
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Yes
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Components
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| • | 55 µl | Clonables 2X Ligation Premix
| | • | 10 µl | Clonables Positive Control
| | • | 1.5 ml | Nuclease-free Water
| | • | 11 × 50 µl | NovaBlue Singles™ Competent Cells
| | • | 2 × 2 ml | SOC Medium
| | • | 10 µl | Test Plasmid |
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Features and benefits
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Fast! 15-minute ligation, 8-minute transformation with ampicillin selection
Ligation components premixed to decrease pipetting steps and increase reliability
One reaction, optimized for cohesive ends,
single base overhangs, and blunt ends
Single-use competent cells eliminate need to aliquot, freeze/thaw, or waste partially used vials
Compatible with PCR buffer, TE, restriction
enzyme buffer, or End Conversion Mix
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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