Perfect RNA™ Markers, 0.1-1 kb are a mixture of seven defined RNA transcripts ranging from 100 to 1000 nucleotides, which facilitate the size determination of unknown RNA samples. The markers have uniform band intensities and are spaced at even, easy-to-remember sizes. They are suitable for native or denaturing agarose or polyacrylamide gel analysis of small RNA, especially for RNase protection assays.
For convenient preparation of labeled markers, the Perfect RNA Marker Template Mix, , 0.1–1 kb, (Cat. No.
69003), is also available. the mixture contains optimized amounts of seven linearized plasmid templates, ready for transcription with T7 RNA Polymerase (Cat. No.
69255). In the presence of appropriately labeled nucleotides, a set of labeled Perfect RNA Markers can by synthesized in one reaction.
The RNA Sample Buffer (Cat. No.
70606), which is available separately, is a convenient, premixed, formamide-containing sample buffer for experimental samples and Perfect RNA Markers. This sample buffer is suitable for native and denaturing gels.