The pET-23a-d(+) vectors carry an N-terminal T7•Tag
® sequence plus an optional C-terminal His•Tag
® sequence. These vectors differ from pET-21a-d(+) by the "plain" T7 promoter instead of the T7
lac promoter and by the absence of the
lacI gene. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (
TB051). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.