Bacteriophage CE6 is a recombinant lambda phage containing the T7 RNA polymerase gene (Studier 1986). The polymerase gene is cloned into the int gene, such that it is transcribed from the pL and pI promoters of the phage during infection. λCE6 can be used to provide a source of T7 RNA polymerase to susceptible host cells carrying T7 expression plasmids. In general, it is used when a target gene is so toxic that the plasmid cannot be maintained in DE3 lysogenic hosts, or with alternative hosts that are not DE3 lysogens. The phage has λcI857 immunity and the Sam7 mutation, which requires supF hosts for lytic growth (neither lytic growth nor different immunity are required for T7 RNA polymerase synthesis). It is provided as a high titer lysate and includes host strains LE392 (rK12– mK12+) and ED8739 (rK12– mK12–) as glycerol stocks. The 10-ml size is enough to induce up to 100 ml culture.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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Product information
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Avoid freeze/thaw
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Yes
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Components
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| • | 0.2 or 10 ml | CE6 Lysate
| | • | 0.2 ml | LE392 glycerol stock
| | • | 0.2 ml | ED8739 glycerol stock |
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS
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Licence Brookhaven
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This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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