For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
14 February 2012
loading
The Perfect Protein™ Markers are a novel set of recombinant proteins with defined
sizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gel
electrophoresis, the Perfect Protein Markers enable highly accurate size determination of
unknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75, 100, and 150 kDa. Each vial contains 400 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75, 100, and 150 kDa. Each vial contains 400 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).
The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
| Related products | |
|---|---|
| 69149 | Perfect Protein™ Markers, 15-150 kDa |
| 71614 | Strep•Tag® II Perfect Protein™ Markers, 16–100 kDa, Lyophilized |
| Related products for Perfect Protein™ Markers, 10-225 kDa | |
| 69149 | Perfect Protein™ Markers, 15-150 kDa |
| 71614 | Strep•Tag® II Perfect Protein™ Markers, 16–100 kDa, Lyophilized |
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Avoid freeze/thaw | Yes | ||
| Features and benefits |
• Markers are supplied at the working dilution in
gel loading buffer; concentration optimized for
Coomassie blue staining • Recombinant, unmodified markers produce sharp, accurately sized bands • Available in two size ranges: 15-150 kDa 10-225 kDa |
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| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Toxic |
||
