The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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Product information
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Avoid freeze/thaw
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Yes
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Components
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| • | 1 µg | Ek/LIC Vector
| | • | 10 µl | Ek/LIC Control Insert
| | • | 25 U | T4 DNA Polymerase, LIC-qualified
| | • | 50 µl | 10X T4 DNA Polymerase Buffer
| | • | 100 µl | 100 mM DTT
| | • | 50 µl | 25 mM EDTA
| | • | 40 µl | 25 mM dATP
| | • | 1.5 ml | Nuclease-free Water
| | • | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells
| | • | 0.2 ml | BL21(DE3) Competent Cells
| | • | 0.2 ml | BL21(DE3)pLysS Competent Cells
| | • | 5 × 2 ml | SOC Medium
| | • | 10 µl | Test Plasmid |
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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Licence Brookhaven
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This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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Data
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