The Thrombin Cleavage Capture Kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of Biotinylated Thrombin for digestion and its subsequent removal with Streptavidin Agarose. The kit is suitable for use with any fusion protein that contains a thrombin recognition sequence. A Cleavage Control Protein is included in the kit to monitor performance of cleavage conditions. It is cleaved into 2 fragments, which are easily visualized by SDS-PAGE.
The Cleavage Control Protein is also available separately to monitor performance of either thrombin or enterokinase cleavage conditions. The 48 kDa control protein is cleaved into two proteolytic fragments of 35 kDa and 13 kDa, which are easily visualized by SDS-PAGE.
| Product information | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Avoid freeze/thaw | Yes | ||||||||||||||||||||
| Components |
|
||||||||||||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | Multiple storage requirements | ||
| Ship |
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS |
||
| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
||
| R Phrase |
R: 36/37/38 |
||
