x

Welcome to Merck Chemicals

 

Please choose your country:

North America

Latin America

Europe

Africa

Asia / Pacific

All countries from A to Z

If you can't find your country please click here.

Logo Merck KGaA
Merck4Biosciences | International

Important message


It is currently not possible to order Bioscience products together with other Merck products. We recommend completing your current order first.

Do you wish to proceed without completing your current order? If so, this will empty your shopping cart.


Information
X


Merck4Biosciences

Novabiochem

Novagen

Calbiochem


69001  First Strand cDNA Synthesis Kit

Reliable preparation of templates for RT-PCR

The First Strand cDNA Synthesis Kit is designed for the preparation of high quality first strand cDNA from cellular RNA templates. The kit contains MMLV Reverse Transcriptase for superior yields of full-length cDNA. Both oligo(dT) and random hexamer primers are included for a choice of general priming strategies and as alternatives to user-supplied specific primers. A small volume (1-2 µl) of the first strand cDNA reaction can be used in PCR amplification with KOD Hot Start or KOD XL DNA Polymerase. Use this kit in conjunction with the Straight A’s™ mRNA Isolation System and appropriate PCR reagents to amplify rare coding regions.
Product number Size Quantity Price
69001-3  40 rxn  price on request 
Product information
Avoid freeze/thaw Yes
Components
4000 UMMLV Reverse Transcriptase
200 µl5X First Strand Buffer
100 µl100 mM DTT
50 µl10 mM dNTP Mix
20 µgOligo(dT) Primer
10 µgRandom Hexamer Primers
1.5 mlNuclease-free Water
1 µgPositive Control RNA
10 µlPositive Control Primers, 3' AS
10 µlPositive Control Primers, 5' S
Store and ship information
Storage Multiple storage requirements
Ship Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS
Safety information
S Phrase S: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
R Phrase R: 22-36/38

Harmful if swallowed.

Top

© Merck KGaA, Darmstadt, Germany, 2010