662053
UbcH3 Conjugating Enzyme E2, His•Tag®, Human, Recombinant, E. coli
CDC34, UBE2R2
Full-length, recombinant, human UbcH3 fused at the N-terminus to His•Tag® sequence and expressed in E. coli. UbcH3, an E2 ubiquitin conjugase homologous to Cdc34 in yeast, is important in the control of cell cycle and DNA replication. UbcH3/Cdc34 in association with different E3 complexes, including SCF, has been shown to target proteins including IκB, B-Myb, Wee1, MyoD, ICERIIγ, ATF5, p27Xic1, and p27Kip1. Other targets such as β-catenin, p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of Cdc34 by virtue of their SCF requirement for proteolysis. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
| Product information | |||
|---|---|---|---|
| Formulation | In 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 8.0. | ||
| Molar mass | 27000 | ||
| Avoid freeze/thaw | Yes | ||
| Purity | ≥95% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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