The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC). The identification of phosphorylation sites is routinely accomplished by mass spectrometry. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis.
| Product information | |||
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| Format | Phosphopeptide enrichment | ||
| Form | 100 Isolations | ||
| Detection method | Mass Spectrometry | ||
| Kit contains | 10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol. | ||
| Store and ship information | |||
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | +2°C to +8°C | ||
| Ship |
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
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 <b>The combination of SCX and IMAC exhibits high selectivity for phosphopeptides by reducing nonspecific binding of peptides from complex mixtures.</b> A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides. The samples were subsequently processed using (A) an ironoxide-based IMAC material alone or (B) the combination of SCX with zirconium-based IMAC particles as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode. |
