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14 February 2012
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Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
| Alternatives | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| Alternative products for PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹80, pTyr¹82) Rabbit pAb | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| Accessories forPhosphoDetect™ Anti-p38 MAP Kinase (pThr¹80, pTyr¹82) Rabbit pAb |
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹80 and Tyr¹82 phosphorylation sites of human p38 MAP Kinase | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3. | ||
| Species reactivity | canine, human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | UV irradiated HEK293 cells or sorbitol-treated PC12 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human p38 MAP kinase phosphorylated at Thr<sup>180</sup>/Tyr<sup>182</sup>, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr<sup>180</sup>, pTyr<sup>182</sup>) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence. |
