Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
| Accessories for PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹80, pTyr¹8²) Rabbit pAb |
|
Product information
|
|
Host
|
Rabbit
|
|
Isotype
|
IgG
|
|
Immunogen
|
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹80 and Tyr¹8² phosphorylation sites of human p38 MAP Kinase
|
|
Form
|
Liquid
|
|
Formulation
|
In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
|
|
Species reactivity
|
canine, human, mouse, rat
|
|
Preservative
|
≤0.1% sodium azide
|
|
Applications
|
-
Immunocytochemistry
-
Immunoblotting (Western Blotting)
|
|
Avoid freeze/thaw
|
Yes
|
|
Positive control
|
UV irradiated HEK293 cells or sorbitol-treated PC12 cells
|
|
Store and ship information
|
|
|
|
Storage
|
-20°C
|
|
Ship
|
Dry Ice Only
Standard Handling
|
|
Canadian export regulations
|
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
|
|
Data
|
Detection of human p38 MAP kinase phosphorylated at Thr<sup>180</sup>/Tyr<sup>182</sup>, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr<sup>180</sup>, pTyr<sup>182</sup>) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
|
