Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.
Potency: ≥1000 µ/mg solid.
| Product information | |||
|---|---|---|---|
| Form | Light brown lyophilized solid | ||
| Molar mass | 527.5 | ||
| Biological activity | The activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard. | ||
| Purity | ≥90% by HPLC and TLC | ||
| Solubility | dH2O | ||
| Chemical formula | C20H37N3O13 | ||
| RTECS | WK2130000 | ||
| CAS number | 31282-04-9 | ||
| Merck USA index | 14, 4852 | ||
| Store and ship information | |||
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | +2°C to +8°C | ||
| Ship |
Ambient Temperature Only
Highly Toxic & Carcinogenic / Teratogenic |
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| Safety information | |||
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| S Phrase |
S: 22-26-36/37/39-45 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 26/27/28-37/38-41 Risk of serious damage to eyes. |
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