Recognizes the ~34 kDa Cdc2/Cdk1 protein phosphorylated at Tyr15 in rat brain.
| Accessories for PhosphoDetect™ Anti-Cdc2 (pTyr¹5) Rabbit pAb |
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Product information
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Host
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Rabbit
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Isotype
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IgG
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Immunogen
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a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹5 phosphorylation site in rat Cdc2/Cdk1
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Form
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Liquid
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Formulation
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In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
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Species reactivity
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rat
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Preservative
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None
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Applications
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Dot Blot
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Immunoblotting (Western Blotting)
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Avoid freeze/thaw
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Yes
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Store and ship information
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Storage
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-20°C
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Ship
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Dry Ice Only
Standard Handling
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Data
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Detection of human Cdc2 phosphorylated on Tyr<sup>15</sup> by immunoblotting. Samples: Whole cell lysate from Human T47D untreated (lane 1) with treatment from EGF (lane 2) and lambda-phosphatase (lanes 3 and 4). Primary antibody: PhosphoDetect™ Anti-Cdc2 (pTyr<sup>15</sup>) Rabbit pAb (Cat. No. 219437) (1:1000). Detection: chemiluminescence.
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