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Merck4Biosciences (バイオ)

WideScreen®

Calbiochem

Novabiochem

Novagen

70072  pET-32 Xa/LIC Vector Kit

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153-8927 東京
Japan
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Fax: 0120-189-350
E-mail mj_service@merckgroup.com

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13 2月 2012

ローディング
 
注文番号 包装サイズ 数量 希望販売価格
70072-3  20 rxn 
ローディング
価格は変更する可能性があります。
The pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
製品情報
Avoid freeze/thaw はい
Components
1 µgXa/LIC Vector
10 µlXa/LIC Control Insert
25 UT4 DNA Polymerase, LIC-qualified
50 µl10X T4 DNA Polymerase Buffer
100 µl100 mM DTT
50 µl25 mM EDTA
40 µl25 mM dGTP
1.5 mlNuclease-free Water
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
0.2 mlBL21(DE3) Competent Cells
0.2 mlBL21(DE3)pLysS Competent Cells
5 × 2 mlSOC Medium
10 µlTest Plasmid
保管と送付情報
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

© Merck KGaA, Darmstadt, Germany, 2012

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