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13 Fevereiro 2012
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| Nº de produto | Tamanho |
|---|---|
| PK1015-50UG | 50 μg |
Recognizes the ~60 kDa p70S6 kinase protein phosphorylated at Tyr389 in MCF7 cells.
| Informações sobre o produto | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Thr389 phosphorylation site of human p70S6K | ||
| Form | Liquid | ||
| Formulation | In Tris citrate/phosphate buffer, pH 7-8. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
|
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| Avoid freeze/thaw | Sim | ||
| Positive control | MCF7 cells | ||
| Informação de estoque e transporte | |||
|---|---|---|---|
| Armazenagem | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
| Dados | |||
|---|---|---|---|
 Detection of human p70S6 kinase, phosphorylated on Thr<sup>389</sup>, by immunoblotting. Samples: Whole cell lysate (50 µg) from MCF7 cells left untreated (lane 2,4) or treated with 100 nM rapamycin for 15 min (lane 3,5); lane 4 and 5: lysates treated with lambda phosphatase. Primary antibody: PhosphoDetect™ Anti-p70S6K (pThr<sup>389</sup>) Rabbit pAb (Cat. No. PK1015) (1 µg/ml). Detection: chemiluminescence. |
