PS1020
PhosphoDetect™ Anti-mTOR (pSer2448) Rabbit pAb
Anti-RAFT1, Anti-FRAP2, Anti-FRAP1, Anti-FK506 binding protein 12-rapamycin associated protein 1, Anti-RAPT1
Para informações, por favor, contate o nosso Serviço de Atendimento ao Cliente
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefone: +49 6151 72-0
Fax: +49 6151 72 2000
13 Fevereiro 2012
carregando
| Nº de produto | Tamanho |
|---|---|
| PS1020-50UG | 50 μg |
Recognizes the ~290 kDa mTOR protein phosphorylated at Ser2448 in EGF-treated HEK293 cells.
| Informações sobre o produto | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser2448 phosphorylation site of human TOR | ||
| Form | Liquid | ||
| Formulation | In Tris citrate/phosphate buffer, pH 7.0-8.0. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Sim | ||
| Positive control | EGF-treated HEK293 cells | ||
| Informação de estoque e transporte | |||
|---|---|---|---|
| Armazenagem | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Dados | |||
|---|---|---|---|
 Detection of human TOR, phosphorylated at Ser<sup>2448</sup>, by immunoblotting. Samples: Whole cell lysate (50 µg) from EGF-treated HEK293 cells incubated without lambda phosphatase (lanes 1,2) or with lambda phosphatase (Cat. No. 539514) (lane 3). Primary antibody: Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) (Cat. No. OP97) (3 µg/ml) (lane 1) and PhosphoDetect™ Anti-mTOR (pSer<sup>2448</sup>) Rabbit pAb (Cat. No. PS1020) (3 µg/ml) (lanes 2,3). Detection: chemiluminescence. |
